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Pickart and colleagues reported in Nature (1980) that the tripeptide facilitates copper uptake into cultured cells, and potentiometric titration places the GHK-Cu stability constant at log K of approximately 16.4, marginally above the copper transport site of human serum albumin at roughly 16.2

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Common Protocol Mistakes We've Seen Over the years, our team has consulted on countless research projects, and we've seen a few common missteps that can inadvertently sabotage results

Next, it was incubated with horseradish peroxidase-conjugated species-specific secondary antibody for 1 h and then washed for three times before further analysis